Selection against spermatozoa with fragmented DNA after postovulatory mating depends on the type of damage
© Hourcade et al; licensee BioMed Central Ltd. 2010
Received: 2 December 2009
Accepted: 31 January 2010
Published: 31 January 2010
Before ovulation, sperm-oviduct interaction mechanisms may act as checkpoint for the selection of fertilizing spermatozoa in mammals. Postovulatory mating does not allow the sperm to attach to the oviduct, and spermatozoa may only undergo some selection processes during the transport through the female reproductive tract and/or during the zona pellucida (ZP) binding/penetration.
We have induced DNA damage in spermatozoa by two treatments, (a) a scrotal heat treatment (42 degrees C, 30 min) and (b) irradiation with 137Cs gamma-rays (4 Gy, 1.25 Gy/min). The effects of the treatments were analyzed 21-25 days post heat stress or gamma-radiation. Postovulatory females mated either with treated or control males were sacrificed at Day 14 of pregnancy, and numbers of fetuses and resorptions were recorded.
Both treatments decreased significantly implantation rates however, the proportion of fetuses/resorptions was only reduced in those females mated to males exposed to radiation, indicating a selection favoring fertilization of sperm with unfragmented DNA on the heat treatment group. To determine if DNA integrity is one of the keys of spermatozoa selection after postovulatory mating, we analyzed sperm DNA fragmentation by COMET assay in: a) sperm recovered from mouse epididymides; b) sperm recovered from three different regions of female uterine horns after mating; and c) sperm attached to the ZP after in vitro fertilization (IVF). Similar results were found for control and both treatments, COMET values decreased significantly during the transit from the uterine section close to the uterotubal junction to the oviduct, and in the spermatozoa attached to ZP. However, fertilization by IVF and intracytoplasmatic sperm injection (ICSI) showed that during sperm ZP-penetration, a stringent selection against fragmented-DNA sperm is carried out when the damage was induced by heat stress, but not when DNA fragmentation was induced by radiation.
Our results indicate that in postovulatory mating there is a preliminary general selection mechanism against spermatozoa with low motility and fragmented-DNA during the transport through the female reproductive tract and in the ZP binding, but the ability of the ZP to prevent fertilization by fragmented-DNA spermatozoa is achieved during sperm-ZP penetration, and depends on the source of damage.
Sperm DNA damage is gaining interest as a potential cause of infertility, and it may be initiated by a wide range of causes: drugs, chemotherapy, radiation therapy, cigarette smoking and environmental toxins, genital tract inflammation, testicular hyperthermia, varicoceles, hormonal factors, etc (Reviewed in ). The normality of sperm nuclear DNA plays a critical role in mammalian fertilization and subsequent embryonic development. It has been demonstrated that sperm cells with damaged or fragmented-DNA can fertilize oocytes in vitro. Some authors consider that this also happens in vivo and that highly motile mouse sperm did not differ in types and frequencies of chromosomal abnormalities from those not selected for motility . However, it has been reported in humans that swim-up selection based on sperm motility excludes many sperm with ultrastructural evidence of apoptosis, as confirmed by TUNEL analysis ; also there is a correlation between sperm motility and sperm chromatin structure assay (SCSA) parameters ; and that sperm DNA fragmentation affects sperm motility and fertilization rates . It has been reported in vivo that the likelihood of boar spermatozoa with unstable chromatin to reach and to fertilize the oocyte is very low . There is evidence suggesting that the journey of the sperm cells from the site of deposition to the site of fertilization is both dynamic (by the sperm and the female tract) and highly complex . Passage of sperm through the female reproductive tract is regulated to maximize the chance of fertilization and to ensure that sperm cells with normal morphology and vigorous motility will be the ones to succeed .
Regardless of the large number of spermatozoa in an ejaculate, only a minority are able to meet the stringent requirements needed to fertilize the oocyte. This reduction should be due to the presence of mechanisms within the female reproductive tract that act as checkpoints for the selection of fertilizing spermatozoa. The three principal mechanisms are (a) the female reproductive tract microenvironment and fluids molecules; (b) the sperm-oviduct (caudal isthmus) interaction (a pre-ovulatory mechanism); and (c) the sperm-zone pellucida interaction (a post-ovulatory mechanism) . It is accepted that adhesion to the isthmus plays a key role in sperm selection and storage of sperm until ovulation. However, mating after ovulation impedes sperm attachment to the oviduct, reducing the female sperm selection mechanisms. In this case, only the sperm-zona pellucida (ZP) interaction may produce selection of the spermatozoa. In fact, it has been suggested that in humans, the binding of spermatozoa to ZP selects spermatozoa endowed with progressive motility, normal morphology and chromatin structure , and may also discriminate against spermatozoa with numerical chromosomal aberrations . It has been reported that in vitro, sperm with single stranded or denatured DNA bind less or do not bind at all to the ZP . In pigs, spermatozoa with stable chromatin are more likely both to bind to the oviduct and to traverse the reproductive tract in vivo, ultimately reaching the oocytes and penetrating the zona pellucida. Since the female reproductive tract does not assess the sperm DNA quality directly, the selection has to be based on sperm phenotype and function [14, 15].
It is not known whether and to what extent chromatin fragmented sperm contributes to the fertilization process in vivo, where selective barriers of the female tract have to be overcome before the sperm meets the oocyte This is the first study to investigate in a post-ovulatory mating model, the selection mechanisms that operate in nature to find out if these mechanisms can discriminate the quality of spermatozoa DNA regardless of the origin of damage.
Experimental design of the in vivo experiments
All experimental procedures were approved by our Institutional Review Board according to the Guide for Care and Use of Laboratory Animals as adopted by the Society for Study of Reproduction. Mice were kept on a 14L:10D light cycle. CD1 female mice (8-10 wk old) were paired with male mice of the same strain to allow mating. Adult males CD-1 (Harlan, Oxon; UK), 12 weeks old, were used for the heat stress (n = 8) and γ-radiation (n = 9) experiments. Scrotal heat stress was realized as previously described . Briefly, males were anesthetized with an intraperitoneal injection of 0.1 ml/10 g body weight of a solution containing 10 mg of ketamine and 1 mg of xylazine per ml. A cotton string was loosely applied around the scrotum to avoid retraction of the testes to an abdominal position, without constraining testicular vascularization. The males were then passed through a hole in a polystyrene "raft" up to the mid-body level, so that they floated on the water surface with their lower body and testes immersed in water at 42°C for 30 min. After returning to their cages, the animals were placed on a warm mat (25°C) to maintain their body temperature until they had fully recovered from the anesthesia. Control males (n = 5) were also anesthetized and placed in a water bath at 33°C for 30 min and recovered in the same way .
Sperm counts from non-treated and treated males, collected from vas deferens, as well as sperm counts from different uterine sections were performed using a Bürke hemocytometer. Motility was determined by loading a sperm sample onto a pre-warmed (37°C) slide and placing it on the heated (37°C) microscope stage. Percentages of motile spermatozoa were assessed by subjective analysis of two different observers in at least 3 different fields. Since no statistical differences were found among data from the 2 control groups (sham irradiated and submitted to 33° water bath), we combined them into a single control group for further comparisons.
Experimental design of the in vitro experiments
In vitro fertilization (IVF) was performed as described in . Oocytes were collected from superovulated CD1 female mice (8-10 wk old). Superovulation was induced by intraperitoneal injections of 7.5 IU of equine chorionic gonadotropin (eCG; Intervet, Boxmeer, Holland), followed 48 h later by 5 IU of human CG (hCG; Lepori, Farma-Lepori, Barcelona, Spain) . Cumulus-oocytes-complexes (COCs) were obtained from the ampulla and cumulus cells were removed by incubation in hyaluronidase in M2 medium. Groups of. 20-30 oocytes were placed in each fertilization drop of 500 μl of Human Tubal Fluid medium (HTF) supplemented with BSA and covered with mineral oil. Sperm was collected from adult CD-1 males subjected to scrotal heating and γ-radiation as described for the in vivo experiments. The males were killed by cervical dislocation 21-25 days after treatment. Spermatozoa collected from the cauda and the vas deferent were incubated in HTF drops supplemented with BSA, during 30 min at 37°C with 5% CO2 to achieve capacitation. After incubation, 10-15 μl of the sperm suspension were collected from the peripheral part of the capacitation drop with a pipette tip. This suspension was transferred into the fertilization drop and co-incubated with the oocytes in HTF medium (8 × 105 motile spermatozoa/ml) for 5 hours. Then, oocytes were washed three times and placed in culture drops of potassium simplex optimized medium with amino acids (KSOMaa). Cleavage rate was assessed at 24 hours post insemination and embryo culture was followed to the blastocysts stage.
Intracytoplasmatic sperm injection (ICSI) was performed as described in . Briefly, metaphase II oocytes were collected from 6- to 8- week-old female mice superovulated and cumulus cells were removed with the same procedure used for IVF. Oocytes were washed and maintained in KSOMaa at 37°C in a 5% CO2 in air atmosphere until use. Fresh sperm samples from heat stressed, γ-radiated or control CD1 mice were used to perform ICSI. Sperm was diluted 1:5 with M2 medium containing 10% polyvinyl-pyrrolidone (PVP) to decrease stickiness (1 part sperm: 5 parts M2+PVP). ICSI was performed in M2 medium at room temperature. The ICSI dish contained a manipulation drop (M2 medium), a sperm drop (sperm solution in M2/10% PVP), and an M2/10% PVP needle cleaning drop. Injections were performed with a PMM-150 FU piezo-impact unit (PrimeTech, Tokyo, Japan) and Eppendorf micromanipulators (Hamburg, Germany) using a blunt-ended mercury-containing pipette with 6 to 7 mm of inner diameter . The head of the fresh sperm cell was separated from the midpiece and tail by applying 1 or more piezoelectric pulses. Groups of 10 oocytes were injected with individual sperm heads. After 15 minutes of recovery at room temperature in M2 medium, surviving oocytes were washed 3 times in equilibrated KSOMaa and returned to mineral oil-covered KSOMaa drops and cultured at 37°C in a 5% CO2 air atmosphere. Cleavage was assessed at 24 hours post sperm injection and cleaved embryos were cultured in vitro until blastocyst.
Collection of sperm samples from the in vitro fertilization experiment for DNA fragmentation analysis
Two hours after IVF all sperm bound to the surface of ZPs were removed by repeated vigorous aspiration with a narrow pipette with an inner diameter slightly smaller than the oocyte in a 35 mm-dish with 30 μl Phosphate Buffered Saline (PBS) supplemented with 0.5% Bovine Serum Albumin (BSA). Since only a small number of spermatozoa was bound to ZP in some experimental groups, 25 oocytes were used for each one. DNA damage of removed ZP-bound spermatozoa and spermatozoa retrieved from different uterine sections and males (control, heat stressed and γ-radiated) as described in in vivo experiment (see above) were assessed by single-cell gel electrophoresis (SCGE or Comet assay), following the protocol described in . Analysis of the shape and length of "comet" tail, just like the DNA content in the tail, gives an assessment of DNA damage. Briefly, 30 μl-sperm suspension (10000 spermatozoa/μl each sample except oviduct samples in which concentration was 100 spermatozoa/μl) was diluted in low-melting-point agarose (70 μl, 1% w/v; LM-2, Hispanlab, Madrid, Spain). A 100-μl mixture of sperm-agarose was immediately pipetted onto agarose-coated slides (1% w/v normal-melting-point agarose). Samples were immersed in ice-cold lysing solution (Trevigen) supplemented with 40 mM dithiotreitol and proteinase K (200 μg/ml). Incubation was performed during 1 h at 37°C. After this step, slides were rinsed in distilled water three times (5 min each time) and incubated in electrophoresis neutral buffer (Tris-borate-EDTA, pH 8) for 20 min. Electrophoresis was then performed at 25 V and 300 mA for 7 min. Following electrophoresis, the slides were neutralized with Tris-HCl buffer (pH 7.4) for 5 min and rinsed in distilled water. Samples were stained with SYBR Green (Trevigen) and analyzed under an epifluorescence microscope (Optiphot-2; Nikon). Comets were analyzed using specialized SCGE analysis software (CometScore, freeware version; TriTek Corp). Tail length (pixels, px) and tail moment (arbitrary units, A.U.) were recorded in 100 cells per sample. Both parameters allowed us to describe extension of DNA damage. Tail length indicates the distance migrated. Tail moment is the product of the tail length and the fraction of DNA in the tail, calculated by the analysis software. It was considered as an indirect measure of the size of the fragments generated. In order to evaluate DNA damage in spermatozoa in terms of single strand breaks, we used alkaline gel electrophoresis as previously described . Briefly, DNA was incubated at 55°C overnight in lysis buffer (50 mM NaCl, 10 mM Tris pH 8.0, 1 mM EDTA, pH 8.0) containing 1% SDS, 1% (v/v). b-mercaptoethanol and 2 mg/ml proteinase K. DNA was isolated by phenol/chloroform extraction, were quantify and 10 μg of DNA were loaded in each gel well.
Scrotal heat stress, γ-radiated and control mice were killed by cervical dislocation, and the testes were fixed in Davidson Fluid modified  for 48 h, then rinsed with PBS, and stored in 70% ethanol until analysis. Entire testes were embedded in paraffin using routine histologic procedures for subsequent light microscopic evaluation. Serial sections measuring 10 μm in thickness were then cut from the paraffin blocks and selected for staining. Ten sections were taken from every mouse testis. Finally, all sections were stained with hematoxylin/eosin. Two investigators blinded to the groups interpreted structural changes. At least 8 animals per treatment were analyzed.
Statistical analyses were performed using SigmaStat version 3.1.1 software (Jandel Scientific, San Rafael, CA). Data are given as the mean ± S.E.M. Comparison of the differences between the means for each treatment were done using ANOVA followed by Student-Newman-Kleus post hoc test.
Effect of treatment on morphology of seminiferous tubules and sperm characteristics
DNA fragmentation was induced by scrotal heat stress and γ-radiation. Based on previous results  we analyzed the effect of the treatments 21-25 days post heat stress o γ-radiation in order to asses the effect of such insult on the spermatocytes at the time of treatment, one of the more sensitive stages of spermatogenesis, when a higher DNA fragmentation is induced [16, 27]. 21-25 days is the estimated time that spermatocytes need to become mature spermatozoa.
Effects of treatment with heat on body weight, testis weight, epidydimal weight and sperm characteristics - motility and sperm counts (ANOVA analysis)
(n = 9)
Scrotal Heat Stress
(n = 8)
(n = 10)
Body weight (g)
37.06 ± 1.69
35.96 ± 0.73
36.72 ± 0.74
Testis weight (mg)
239 ± 14a
138 ± 10b
127 ± 5b
Testis weight per body weight (mg/g)
6.06 ± 0.88a
2.54 ± 0.36b
3.47 ± 0.13b
Epididymal weight (mg)
133.0 ± 10.5
113. 5 ± 10.7
133 ± 27
Vas deferens sperm counts (×106)
11.6 ± 0.8a
4.4 ± 1.7b
7.6 ± 1.3c
69 ± 3a
21 ± 5b
52 ± 4c
Effect of treatment on implantation, resorptions, pregnancies and quality of embryo produced in vivo
DNA integrity of sperm recovered from uterus, oviduct, and sperm attached to the zona pellucida
Effect of scrotal heat stress and γ-radiation on Tail length on Comets from spermatozoa recovered from different sections along uterine tract and assessed by neutral Comet assay
6.71 ± 0.82aα
30.94 ± 5.62b
25.71 ± 2.42b
8.86 ± 0.8a
(n = 9)
Scrotal Heat Stress
38.96 ± 4.09aβ
38.37 ± 4.66a
29.39 ± 3.52a
11.20 ± 1.50b
(n = 9)
17.20 ± 3.58aγ
44.58 ± 12.08b
32.38 ± 9.58ab
11.18 ± 2.99b
(n = 8)
Effect of scrotal heat stress and γ-radiation on Tail length on Comets from spermatozoa recovered after in vitro fertilization from zona pellucida and assessed by neutral Comet assay
16.20 ± 1.02aα
14.76 ± 3.98a
4.55 ± 0.56aα
3.72 ± 1.30a
(n = 9)
Scrotal Heat Stress
26.64 ± 3.34aβ
15.96 ± 2.92b
14.82 ± 2.34aβ
8.96 ± 3.31b
(n = 9)
46.95 ± 6.55aγ
21.43 ± 5.84b
29.90 ± 6.34aγ
8.68 ± 3.15b
(n = 8)
Consequences of the use of spermatozoa from treated males for IVF and ICSI in embryo preimplantation development
Cleavage rate and development of oocytes fertilized by In Vitro fertilization and ICSI with non-treated, scrotal heat stressed and γ-radiated spermatozoa
Total oocytes (experiments)
2-cell embryos (%)
Scrotal Heat Stress
Injected Oocytes (experiments)
Surviving Oocytes (%)
Scrotal Heat Stress
When natural barriers of fertilization were overcome by ICSI, number of surviving oocytes was not affected by the source of spermatozoa (control, heat-stressed or radiated), but both cleavage rate and blastocyst developmental rate of the control group was significantly higher than in the heat stressed and radiated groups, suggesting a lower DNA quality of these spermatozoa.
The female reproductive tract holds the key to mammalian sperm selection. After mating there is a preselection of spermatozoa based on their motility, and those that successfully overcome this selection become sequestered within the oviductal sperm reservoir until ovulation takes place, then spermatozoa are untied and proceed towards the oocytes. When mating takes place after ovulation, spermatozoa only may undergo some selection processes during the transport through the female reproductive tract and/or during the zona pellucida (ZP) binding/penetration, since there is no sperm attachment to the oviduct. The aim of this work was to investigate in a post-ovulatory mating mice model (mating 6-8 hours after ovulation), the selection mechanisms that operate in nature and to find out if these mechanisms can discriminate the quality of spermatozoal DNA regardless the source of damage; a heat stress process, or a gamma radiation process. Our results indicate that in postovulatory mating there is a preliminary general selection mechanism of spermatozoa with undamaged DNA during the transport through the female reproductive tract and in the ZP binding, but the ability of the ZP to recognize fragmented-DNA spermatozoa is achieved during sperm-ZP penetration, and it depends on the source and type of damage, because only DNA-damaged sperm cells by heat stress but not by radiation were selected. The preliminary selection could be a consequence of selection based on motility since DNA fragmentation and sperm motility have been related . Here, we have demonstrated that higher motile sperm cells that reach the oviduct have lower DNA fragmentation that sperm with lower motility present in the uterus. However, sperm with some level of DNA damage is able to reach the oviduct and to attach to the ZP. Based on these findings the sperm-ZP selection during penetration should be a specific mechanism, because it is only active when sperm-DNA has been fragmented by effect of heat stress and it is not when the damage has been produced by radiation. It has been suggested that stable chromatin structure provides sperm with a rigidity that enables them to penetrate the zona pellucida .
The damage induced in the testes, and the nature and degree of sperm DNA-damage produced by scrotal heat stress or by γ-radiation seems different and may influence the sperm fertilizing capacity. Heat stress decreased testis weight, sperm counts retrieved from vas deferens and motility as described by other authors . In case of radiation, a decrease of testis weight was also observed, a situation that has been reported by other authors ; however, the decrease in number of spermatozoa and sperm motility was less pronounced than with the heat stress. In agreement with previous reports [16, 31, 32], scrotal heat stress produced changes in testicular architecture and DNA-damage of sperm. Histology of testes indicated similar reduction of spermatogenesis in both treated groups; however, in the radiated group it is observed hyperplasia of Leydig cells that might be a consequence of a supraphysiologic hormonal stimulation of the testes originated by the radiation treatment of the male mouse. At higher doses of radiation, a significant loss of primary spermatocytes has been reported . High-temperature in testis is associated with an increase of oxygen free-radical species, which reacts quickly with substrates as lipids, causing a lipid peroxidation that affects spermatozoa membranes . Next to these effects, mitochondrial membrane can be disrupted in spermatozoa and germ cells [35, 36] activating programmed cell death pathways. Gamma radiation tends to cause damage on DNA strands directly (ds or ss breaks) , and more specifically, mutations and alterations of DNA backbone (crosslinking among base pairs, presence of apurinic or apyrimidinic sites) . Alterations in DNA backbone could alter level of chromatin condensation and nuclear volume , parameters related with susceptibility to damage by radiation (Hawkins, 2005). The assay that we have used for quantifying sperm DNA-fragmentation can not determine this second type of mutations produced by the radiation.
Motility is the main factor that allows the sperm to reach the oviduct through the cervix and the utero tubal junction  but it seems that motility is not the unique factor of the sperm selection mechanisms; chemical barriers (e.g., low pH and viscous mucus) and leuckocytic/phagocytotic responses within the female, determine that only a small proportion of the sperm cells deposed in the female tract ever have the opportunity to encounter an oocyte. During some phases of sperm transport through the female reproductive tract, sperms are subjected to physical stresses, are exposed to factors with cell signaling capabilities, and may sustain oxidative damage to their plasma membrane lipids. The initial stages of sperm transport are also mediated by the female tract, because sperm transport is regulated by a combination of intrinsic sperm motility and peristaltic movements of the female reproductive tract. We have found that sperm DNA fragmentation level in spermatozoa recovered from uterus increased compared to sperm DNA fragmentation observed in vas deferens spermatozoa, both in control and in gamma radiated groups. In heat-stressed group, sperm DNA fragmentation in the uterus was also high, but not higher than the value detected for vas deferens spermatozoa. These observations indicate that some damage in sperm DNA is taking place in the uterus. Two mechanisms could be responsible of this effect. On one hand, immune cells present in the uterine mucosa [41–43] such as neutrophils could extrude their nuclear DNA next to associated proteins to form neutrophil extracellular traps (NETs)  that retain spermatozoa with disturbed motility or plasma membrane. During NETs disaggregation, DNAses present in the seminal plasma [45, 46] can act removing connections between neutrophils, attached sperm and proteins, allowing an efficient cleaning of the reproductive tract. The second mechanism could be the presence of nucleases from seminal fluid affecting spermatozoa located in the uterus [46, 47].
Our results agree with previous reports indicating that the passage thought the utero-tubal junction in vivo, is one of the most important selective points that are involved in a plausible selection mechanism in which motility plays a main role . In addition, we have found that the transit form the uterus to the oviduct is critical to reduce sperm with fragmented-DNA. Spermatozoa from the three groups analyzed arriving at the oviduct showed drastic reduction in DNA-damage. This would indicate that there is a selection mechanism in the female reproductive tract based on sperm motility but that indirectly, ensures that spermatozoa with the most intact DNA arrive to the oviduct probably a consequence of a relationship between motility and DNA-fragmentation. We have analyzed the DNA-fragmentation by COMET of high motile sperm selected in vitro, taking the faster population of spermatozoa that are able to cross thought drops of media and we have found a correlation between high motility and low level of DNA-fragmentation (data non published). Other authors have also suggested this relation between motility and DNA-fragmentation by ex vivo or in vitro approaches [7, 48]. However, we have found that in vivo, fertilization with radiated sperm produced a higher number of resorptions, suggesting that in radiated sperm, several DNA-damages that are carried by motile sperm are selected neither in the female tract nor in the ZP penetration. Collectively, these results suggest that there is a relationship between motility and the sperm with damage on DNA strands (ds or ss breaks), but not with mutations and/or alteration of DNA backbone (crosslinking among base pairs, presence of apurinic or apyrimidinic sites).
To analyze if the ZP binding and/or penetration plays a role in the selection of sperm with fragmented-DNA, we have examined the DNA quality of sperm cells attached to the ZP and the preimplantation development of oocytes fertilized in vitro or by ICSI. Sperm cells from treated mice that are attached to the ZP exhibits lower levels of DNA fragmentation that the non-attached sperm, indicating that either ZP performs a positive selection towards unfragmented DNA sperm or that motile sperm (with lower level of fragmented-DNA) are more capable to attach to the ZP. This agrees with previous findings demonstrating that a high number of spermatozoa with normal chromatin were attached to ZP . Samples taken from the fertilization drop showed that there is an increase in sperm DNA fragmentation levels compared to the levels of fragmentation observed in the vas deferens of the control and radiated groups (data not shown). This fact could be explained by the presence of endonucleases delivered from dead sperm cells during IVF [49–51]. Some reports have suggested that membrane-altered spermatozoa liberate some factors to the media, including endonucleases, that could fragment DNA of spermatozoa with unaltered membranes . Also, an increase in ROS production during IVF could take place, escalating damage on alive spermatozoa . In addition, IVF experiments showed that sperm form heat stressed mice that are able to fertilize are also able to produce blastocysts, but when the spermatozoa attached to the ZP are delivered by ICSI, blastocysts development is reduced, indicating that the ability of the ZP to select fragmented-DNA spermatozoa is achieved during sperm-ZP penetration. In the case of sperm from radiated mice, blastocyst development is reduced in comparison to control both after IVF or ICSI, indicating that the ZP is unable to select sperm with DNA damage induced by radiation. These findings agree with our in vivo results since many of the blastocysts produced, apparently normal, could implant, but failed to carry on a normal foetal development and were reabsorbed. On the other hand, in vivo results with heat stressed mice indicate that resulting blastocysts after mating are as competent as control blastocysts to develop into live foetuses. Our results of IVF from heat stress males differ from other study where a block of embryonic development was detected using C57BL/6 inbred mice . The differences may be due to the strain of mice, because we have used CD1 outbreed mice, and it is well established that the genetic background of outbreed and inbred strains of mice influence sperm-assessment parameters, resistance and susceptibility to DNA fragmentation, in vitro fertilization rate, and in vitro embryo development rate; moreover, sperm from the C57BL/6 backgrounds is particularly sensitive . For this reason, results from some specific inbred strains should be considered with precaution.
Our results indicated that when mating takes place after ovulation, female reproductive tract and ZP binding/penetration process play an important role not only in selection of sperm with normal motility and morphology, but also with normal chromatin DNA and/or normal DNA structure/function. The selections in the female tract and during ZP-binding are related to the high correlation between normal motility and normal chromatin DNA. Moreover, there is an active mechanism of selection for sperm with unfragmented DNA that is taking place during the ZP-penetration; however, this mechanism can not recognize sperm carrying some DNA abnormalities, as the DNA mutations originated by radiation, suggesting that other damages concomitantly to DNA fragmentation might be triggered by heat stress, but not by radiation, and thus selection should be based on sperm phenotype and/or function associated with these damages. If the selection mechanisms that operate in nature are able to discriminate the quality of spermatozoa, understanding the basis of the naturally imposed selection mechanisms may help to clarify, which of the many laboratory test are likely to be most informative about fertility. This is of crucial importance in certain situations for example when ICSI treatments are performed as assisted reproductive procedure, because ICSI bypasses multiple natural mechanisms, apparently redundant, which have evolved to ensure selection of high quality sperm cells for fertilization . Sperm DNA fragmentation seems to affect embryo post-implantation development in ICSI procedures in human, resulting in pregnancy loss . Our results strongly suggest that for some types of DNA damage, IVF is a preferential system than ICSI, and that ICSI should be performed with high motile sperm to ensure fertilization with less fragmented-DNA spermatozoa.
uterus near oviduct
uterus near cervix
In Vitro fertilization
Intracytoplasmic Sperm injection.
This work was supported by Grant AGL2009-11358 from the Spanish Ministry of Science and Innovation. JD H. was supported by a grant from National Institute of Agricultural Research and Technologies (INIA).
- Oliva R: Protamines and male infertility. Hum Reprod Update. 2006, 12 (4): 417-435. 10.1093/humupd/dml009.View ArticlePubMed
- Ahmadi A, Ng SC: Fertilizing ability of DNA-damaged spermatozoa. J Exp Zool. 1999, 284 (6): 696-704. 10.1002/(SICI)1097-010X(19991101)284:6<696::AID-JEZ11>3.0.CO;2-E.View ArticlePubMed
- Fatehi AN, Bevers MM, Schoevers E, Roelen BA, Colenbrander B, Gadella BM: DNA damage in bovine sperm does not block fertilization and early embryonic development but induces apoptosis after the first cleavages. J Androl. 2006, 27 (2): 176-188. 10.2164/jandrol.04152.View ArticlePubMed
- Estop A, Catala V, Santalo J: Chromosome constitution of highly motile mouse sperm. Mol Reprod Dev. 1990, 27 (2): 168-172. 10.1002/mrd.1080270213.View ArticlePubMed
- Piomboni P, Bruni E, Capitani S, Gambera L, Moretti E, La Marca A, De Leo V, Baccetti B: Ultrastructural and DNA fragmentation analyses in swim-up selected human sperm. Arch Androl. 2006, 52 (1): 51-59. 10.1080/01485010500203741.View ArticlePubMed
- Giwercman A, Richthoff J, Hjollund H, Bonde JP, Jepson K, Frohm B, Spano M: Correlation between sperm motility and sperm chromatin structure assay parameters. Fertil Steril. 2003, 80 (6): 1404-1412. 10.1016/S0015-0282(03)02212-X.View ArticlePubMed
- Huang CC, Lin DP, Tsao HM, Cheng TC, Liu CH, Lee MS: Sperm DNA fragmentation negatively correlates with velocity and fertilization rates but might not affect pregnancy rates. Fertil Steril. 2005, 84 (1): 130-140. 10.1016/j.fertnstert.2004.08.042.View ArticlePubMed
- Ardon F, Helms D, Sahin E, Bollwein H, Topfer-Petersen E, Waberski D: Chromatin-unstable boar spermatozoa have little chance of reaching oocytes in vivo. Reproduction. 2008, 135 (4): 461-470. 10.1530/REP-07-0333.View ArticlePubMed
- Barratt CL, Kirkman-Brown J: Man-made versus female-made environment--will the real capacitation please stand up?. Hum Reprod Update. 2006, 12 (1): 1-2. 10.1093/humupd/dmi051.View ArticlePubMed
- Suarez SS, Pacey AA: Sperm transport in the female reproductive tract. Hum Reprod Update. 2006, 12 (1): 23-37. 10.1093/humupd/dmi047.View ArticlePubMed
- Hoshi K, Katayose H, Yanagida K, Kimura Y, Sato A: The relationship between acridine orange fluorescence of sperm nuclei and the fertilizing ability of human sperm. Fertil Steril. 1996, 66 (4): 634-639.PubMed
- Van Dyk Q, Lanzendorf S, Kolm P, Hodgen GD, Mahony MC: Incidence of aneuploid spermatozoa from subfertile men: selected with motility versus hemizona-bound. Hum Reprod. 2000, 15 (7): 1529-1536. 10.1093/humrep/15.7.1529.View ArticlePubMed
- Liu DY, Baker HW: Human sperm bound to the zona pellucida have normal nuclear chromatin as assessed by acridine orange fluorescence. Hum Reprod. 2007, 22 (6): 1597-1602. 10.1093/humrep/dem044.View ArticlePubMed
- Satake N, Elliott RM, Watson PF, Holt WV: Sperm selection and competition in pigs may be mediated by the differential motility activation and suppression of sperm subpopulations within the oviduct. J Exp Biol. 2006, 209 (Pt 8): 1560-1572. 10.1242/jeb.02136.View ArticlePubMed
- Holt WV, Van Look KJ: Concepts in sperm heterogeneity, sperm selection and sperm competition as biological foundations for laboratory tests of semen quality. Reproduction. 2004, 127 (5): 527-535. 10.1530/rep.1.00134.View ArticlePubMed
- Perez-Crespo M, Pintado B, Gutierrez-Adan A: Scrotal heat stress effects on sperm viability, sperm DNA integrity, and the offspring sex ratio in mice. Mol Reprod Dev. 2008, 75 (1): 40-47. 10.1002/mrd.20759.View ArticlePubMed
- Baulch JE, Li MW, Raabe OG: Effect of ATM heterozygosity on heritable DNA damage in mice following paternal F0 germline irradiation. Mutat Res. 2007, 616 (1-2): 34-45.View ArticlePubMed
- Yamauchi Y, Ward MA: Preservation of ejaculated mouse spermatozoa from fertile C57BL/6 and infertile Hook1/Hook1 mice collected from the uteri of mated females. Biol Reprod. 2007, 76 (6): 1002-1008. 10.1095/biolreprod.106.059881.View ArticlePubMed
- Kawano N, Yoshida M: Semen-coagulating protein, SVS2, in mouse seminal plasma controls sperm fertility. Biology of reproduction. 2007, 76 (3): 353-361. 10.1095/biolreprod.106.056887.View ArticlePubMed
- Sztein JM, Farley JS, Mobraaten LE: In vitro fertilization with cryopreserved inbred mouse sperm. Biol Reprod. 2000, 63 (6): 1774-1780. 10.1095/biolreprod63.6.1774.View ArticlePubMed
- Gutierrez-Adan A, Pintado B: Effect of flanking matrix attachment regions on the expression of microinjected transgenes during preimplantation development of mouse embryos. Transgenic Res. 2000, 9 (2): 81-89. 10.1023/A:1008926022370.View ArticlePubMed
- Moreira PN, Perez-Crespo M, Ramirez MA, Pozueta J, Montoliu L, Gutierrez-Adan A: Effect of transgene concentration, flanking matrix attachment regions, and RecA-coating on the efficiency of mouse transgenesis mediated by intracytoplasmic sperm injection. Biol Reprod. 2007, 76 (2): 336-343. 10.1095/biolreprod.106.056952.View ArticlePubMed
- Moreira PN, Giraldo P, Cozar P, Pozueta J, Jimenez A, Montoliu L, Gutierrez-Adan A: Efficient generation of transgenic mice with intact yeast artificial chromosomes by intracytoplasmic sperm injection. Biol Reprod. 2004, 71 (6): 1943-1947. 10.1095/biolreprod.104.032904.View ArticlePubMed
- Fernandez-Gonzalez R, Moreira PN, Perez-Crespo M, Sanchez-Martin M, Ramirez MA, Pericuesta E, Bilbao A, Bermejo-Alvarez P, de Dios Hourcade J, de Fonseca FR, Gutierrez-Adan A: Long-term effects of mouse intracytoplasmic sperm injection with DNA-fragmented sperm on health and behavior of adult offspring. Biol Reprod. 2008, 78 (4): 761-772. 10.1095/biolreprod.107.065623.View ArticlePubMed
- Bennetts LE, Aitken RJ: A comparative study of oxidative DNA damage in mammalian spermatozoa. Mol Reprod Dev. 2005, 71 (1): 77-87. 10.1002/mrd.20285.View ArticlePubMed
- Latendresse JR, Warbrittion AR, Jonassen H, Creasy DM: Fixation of testes and eyes using a modified Davidson's fluid: comparison with Bouin's fluid and conventional Davidson's fluid. Toxicol Pathol. 2002, 30 (4): 524-533.View ArticlePubMed
- Haines GA, Hendry JH, Daniel CP, Morris ID: Germ cell and dose-dependent DNA damage measured by the comet assay in murine spermatozoaa after testicular X-irradiation. Biol Reprod. 2002, 67 (3): 854-861. 10.1095/biolreprod.102.004382.View ArticlePubMed
- Bedford JM: Mammalian fertilization misread? Sperm penetration of the eutherian zona pellucida is unlikely to be a lytic event. Biol Reprod. 1998, 59 (6): 1275-1287. 10.1095/biolreprod59.6.1275.View ArticlePubMed
- Gasinska A, Hill S: The effect of hyperthermia on the mouse testis. Neoplasma. 1990, 37 (3): 357-366.PubMed
- Koruji M, Movahedin M, Mowla SJ, Gourabi H, Arfaee AJ: The morphological changes of adult mouse testes after 60Co gamma-Radiation. Iran Biomed J. 2008, 12 (1): 35-42.PubMed
- Rockett JC, Mapp FL, Garges JB, Luft JC, Mori C, Dix DJ: Effects of hyperthermia on spermatogenesis, apoptosis, gene expression, and fertility in adult male mice. Biology of reproduction. 2001, 65 (1): 229-239. 10.1095/biolreprod65.1.229.View ArticlePubMed
- Paul C, Murray AA, Spears N, Saunders PT: A single, mild, transient scrotal heat stress causes DNA damage, subfertility and impairs formation of blastocysts in mice. Reproduction (Cambridge, England). 2008, 136 (1): 73-84.View Article
- Cordelli E, Fresegna AM, Leter G, Eleuteri P, Spano M, Villani P: Evaluation of DNA damage in different stages of mouse spermatogenesis after testicular X irradiation. Radiat Res. 2003, 160 (4): 443-451. 10.1667/RR3053.View ArticlePubMed
- Bansal AK, Bilaspuri GS: Oxidative stress alters membrane sulfhydryl status, lipid and phospholipid contents of crossbred cattle bull spermatozoa. Anim Reprod Sci. 2007
- Silva PF, Gadella BM, Colenbrander B, Roelen BA: Exposure of bovine sperm to pro-oxidants impairs the developmental competence of the embryo after the first cleavage. Theriogenology. 2007, 67 (3): 609-619. 10.1016/j.theriogenology.2006.09.032.View ArticlePubMed
- Sleight SB, Miranda PV, Plaskett NW, Maier B, Lysiak J, Scrable H, Herr JC, Visconti PE: Isolation and proteomic analysis of mouse sperm detergent-resistant membrane fractions: evidence for dissociation of lipid rafts during capacitation. Biol Reprod. 2005, 73 (4): 721-729. 10.1095/biolreprod.105.041533.View ArticlePubMed
- Marchetti F, Essers J, Kanaar R, Wyrobek AJ: Disruption of maternal DNA repair increases sperm-derived chromosomal aberrations. Proc Natl Acad Sci USA. 2007, 104 (45): 17725-17729. 10.1073/pnas.0705257104.PubMed CentralView ArticlePubMed
- Gantchev TG, Girouard S, Dodd DW, Wojciechowski F, Hudson RH, Hunting DJ: Gamma-radiation induced interstrand cross-links in PNA:DNA heteroduplexes. Biochemistry. 2009, 48 (29): 7032-7044. 10.1021/bi9002474.View ArticlePubMed
- Ward WS, Coffey DS: DNA packaging and organization in mammalian spermatozoa: comparison with somatic cells. Biol Reprod. 1991, 44 (4): 569-574. 10.1095/biolreprod44.4.569.View ArticlePubMed
- Nakanishi T, Isotani A, Yamaguchi R, Ikawa M, Baba T, Suarez SS, Okabe M: Selective passage through the uterotubal junction of sperm from a mixed population produced by chimeras of calmegin-knockout and wild-type male mice. Biol Reprod. 2004, 71 (3): 959-965. 10.1095/biolreprod.104.028647.View ArticlePubMed
- Taylor U, Schuberth HJ, Rath D, Michelmann HW, Sauter-Louis C, Zerbe H: Influence of inseminate components on porcine leucocyte migration in vitro and in vivo after pre- and post-ovulatory insemination. Reprod Domest Anim. 2009, 44 (2): 180-188. 10.1111/j.1439-0531.2007.01015.x.View ArticlePubMed
- Taylor U, Rath D, Zerbe H, Schuberth HJ: Interaction of intact porcine spermatozoa with epithelial cells and neutrophilic granulocytes during uterine passage. Reprod Domest Anim. 2008, 43 (2): 166-175. 10.1111/j.1439-0531.2007.00872.x.View ArticlePubMed
- Schuberth HJ, Taylor U, Zerbe H, Waberski D, Hunter R, Rath D: Immunological responses to semen in the female genital tract. Theriogenology. 2008, 70 (8): 1174-1181. 10.1016/j.theriogenology.2008.07.020.View ArticlePubMed
- Alghamdi AS, Foster DN: Seminal DNase frees spermatozoa entangled in neutrophil extracellular traps. Biol Reprod. 2005, 73 (6): 1174-1181. 10.1095/biolreprod.105.045666.View ArticlePubMed
- Szczygiel MA, Ward WS: Combination of dithiothreitol and detergent treatment of spermatozoa causes paternal chromosomal damage. Biol Reprod. 2002, 67 (5): 1532-1537. 10.1095/biolreprod.101.002667.View ArticlePubMed
- Yamauchi Y, Ward MA: Preservation of Ejaculated Mouse Spermatozoa from Fertile C57BL/6 and Infertile Hook1/Hook1 Mice Collected from the Uteri of Mated Females. Biol Reprod. 2007
- Sakkas D, Moffatt O, Manicardi GC, Mariethoz E, Tarozzi N, Bizzaro D: Nature of DNA damage in ejaculated human spermatozoa and the possible involvement of apoptosis. Biol Reprod. 2002, 66 (4): 1061-1067. 10.1095/biolreprod66.4.1061.View ArticlePubMed
- Ramos L, Wetzels AM: Low rates of DNA fragmentation in selected motile human spermatozoa assessed by the TUNEL assay. Hum Reprod. 2001, 16 (8): 1703-1707. 10.1093/humrep/16.8.1703.View ArticlePubMed
- Sotolongo B, Lino E, Ward WS: Ability of hamster spermatozoa to digest their own DNA. Biol Reprod. 2003, 69 (6): 2029-2035. 10.1095/biolreprod.103.020594.View ArticlePubMed
- Sotolongo B, Huang TT, Isenberger E, Ward WS: An endogenous nuclease in hamster, mouse, and human spermatozoa cleaves DNA into loop-sized fragments. J Androl. 2005, 26 (2): 272-280.PubMed
- Shaman JA, Prisztoka R, Ward WS: Topoisomerase IIB and an extracellular nuclease interact to digest sperm DNA in an apoptotic-like manner. Biol Reprod. 2006, 75 (5): 741-748. 10.1095/biolreprod.106.055178.View ArticlePubMed
- Perez-Crespo M, Moreira P, Pintado B, Gutierrez-Adan A: Factors from damaged sperm affect its DNA integrity and its ability to promote embryo implantation in mice. J Androl. 2008, 29 (1): 47-54. 10.2164/jandrol.107.003194.View ArticlePubMed
- Enkhmaa D, Kasai T, Hoshi K: Long-Time Exposure of Mouse Embryos to the Sperm Produces High Levels of Reactive Oxygen Species in Culture Medium and Relates to Poor Embryo Development. Reprod Domest Anim. 2008
- Yildiz C, Ottaviani P, Law N, Ayearst R, Liu L, McKerlie C: Effects of cryopreservation on sperm quality, nuclear DNA integrity, in vitro fertilization, and in vitro embryo development in the mouse. Reproduction. 2007, 133 (3): 585-595. 10.1530/REP-06-0256.View ArticlePubMed
- Borini A, Tarozzi N, Bizzaro D, Bonu MA, Fava L, Flamigni C, Coticchio G: Sperm DNA fragmentation: paternal effect on early post-implantation embryo development in ART. Human reproduction (Oxford, England). 2006, 21 (11): 2876-2881. 10.1093/humrep/del251.View Article
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