Hello, a colleague noted a typographical error that relates to the dose of GnRH-A. I would like to correct that here. Go to section 2 (one page 3) of the methods in paragraph 3, there is a symbol error.
The original sentence is:
"For the priming pre-treatment, females and males were injected i.p. between 14:00- 14:30 with a low dose of GnRH-A (0.04 mg/g; Day 1)".
THE CORRECTED SENTENCE IS :
For the priming pre-treatment, females and males were injected i.p. between 14:00- 14:30 with a low dose of GnRH-A (0.04 MICROGRAMS/g; Day 1).
TO BE CLEAR: Please note that the dose of GnRH-A for priming is 0.04 micrograms per gram body weight not 0.04 millgrams per gram. It is clear that the "micro" symbol is missing.The principle behind this step is to give a lower dose of GnRH-A than that is in the AMPHIPLEX mix. In this way, one can sensitize the pituitary. This is the concept of GnRH priming described in the paper.
I am sorry about this oversight. If you ahve any further questions, please do not hesitate to contact me. Good luck with your experiments. Yours Sincerely, Vance Trudeau (trudeauv@uottawa.ca).
CORRECTION TO PROTOCOL FROM SENIOR AUTHOR
14 March 2014
Hello, a colleague noted a typographical error that relates to the dose of GnRH-A. I would like to correct that here. Go to section 2 (one page 3) of the methods in paragraph 3, there is a symbol error.
The original sentence is:
"For the priming pre-treatment, females and males were injected i.p. between 14:00- 14:30 with a low dose of GnRH-A (0.04 mg/g; Day 1)".
THE CORRECTED SENTENCE IS :
For the priming pre-treatment, females and males were injected i.p. between 14:00- 14:30 with a low dose of GnRH-A (0.04 MICROGRAMS/g; Day 1).
TO BE CLEAR: Please note that the dose of GnRH-A for priming is 0.04 micrograms per gram body weight not 0.04 millgrams per gram. It is clear that the "micro" symbol is missing.The principle behind this step is to give a lower dose of GnRH-A than that is in the AMPHIPLEX mix. In this way, one can sensitize the pituitary. This is the concept of GnRH priming described in the paper.
I am sorry about this oversight. If you ahve any further questions, please do not hesitate to contact me. Good luck with your experiments. Yours Sincerely, Vance Trudeau (trudeauv@uottawa.ca).
Competing interests
None, I am the author.